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RDP FrameBot is a frameshift reworking and close surrounding community member classification tool for use with high-throughput amplicon sequencing. It uses a dynamic programming algorithm to align corresponding query DNA sequence against selected set embracing target protein succession, produces frameshift-corrected protein on top of that DNA sequences and an optimal global or local energy source alignment. The point also helps filter out non-target reads. What online variant belonging to FrameBot is available at http://fungene.cme.msu.edu/FunGenePipeline, tutorial http://rdp.cme.msu.edu/tutorials/framebot/RDPtutorial_FRAMEBOT.html. Developments: FrameBot now uses a kmer pre-filtering heuristic for no-metric-search. This pre-filtering may grow whose speed by one to binary orders of magnitude. CITATION:Wang, Q., Quensen, J. F., Fish, J. A., Lee, TOMORROW. K., Sun, Y., Tiedje, J. M., and Cole, J. RICH. 2013. Ecological designs of nifH genes in four terrestrial climatic zones explored with targeted metagenomics administering FrameBot, a new informatics tool. mBio 4:e00592-13.

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